Single-cell profiling of lncRNA expression during Ebola virus infection in rhesus macaquas

Not bulk tissue analysis but single-cell RNA sequences are necessary for uncovering current gene expression value and tissue specificity. Especially, Ebola virus (EBOV) causes one of most lethal infectious disease and strong inflammatory to humans. Single-cell analysis of circulating monocytes could uncover that correct gene expression value, regulation, and tissue or cell specificity. As the results, they identified 2 times lncRNA by annotating them with single-cell RNA sequences. Expression of lncRNA was in fewer cells than mRNA. Some lncRNA were regulated on EBOV infection. These results show that single-cell RNA sequence is effective for measuring correct lncRNA expression. Some of lncRNA expression can be in only specific cells. LncRNA whose expression is condition or cell-type specific are candidates of disease biomarkers.

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