“Rule of Six”: How Does the Sendai Virus RNA Polymerase Keep Count?

The “Rule of six” stipulates that the Paramyxovirus polymerase efficiently replicate 6n+0 nucleotides genomes especially. Sendai virus (SeV), a member of paramyxovirus, was used through this series of experiments in this study. As the results, SeV RNA polymerase showed selectivity for RNA following to rule of six without C protein. Swap editing site of SeV RNA was effective for length correction. Experiment of constructs with extra 3’-OH sequences showed that the 3’-OH congruence was not an important signal for SeV RNA polymerase. Furthermore, 2 promoters, working independently, were confirmed. Initiation for one promoter was antigenomic promoter, for another was genomic promoter and both obeyed to rule of six. These results showed that the observance of the rule of six can be necessary for polymerase recognition of certain nucleotides properly positioned regarding the nucleoprotein interaction points for appropriate replication.

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