Structural and functional characterization of the severe fever with thrombocytopenia syndrome virus L protein

Severe fever with thrombocytopenia syndrome virus (SFTSV) belongs to the Bunyavirales order. For preventing the bad consequence of this disease, specific vaccine and antiviral drugs are necessary. Viral mRNAs are transcribed by RNA-dependent RNA polymerase L protein that is responsible for the replication of viral genome. So, this is a target point of drug development. This scientific paper revealed the expression and purification procedure of full-length L protein of SFTSV using electron cryo-microscopy and X -ray crystallography. Researcher prevailed 3D models with 70% L protein, polymerase core region, the endonuclease and cap-binding domains. The comprehensive study of structural & biochemical characterization was performed between SFTSV L protein with La Crosse peribunyavirus and influenza orthomyxovirus polymerases to deliver a well-founded structure for futures research of L protein, RNA synthesis and expansion of antiviral strategies against this group.

(AR)